Figure 3.

DNA-independent interaction of PC4 with selected factors. Glutathione-Sepharose 4B beads containing GST-PC4 or GST alone were incubated with the indicated individual factors. After incubation for 30 min on ice and removal of unbound material, the beads were boiled in Laemmli sample buffer. After SDS/PAGE the polypeptides were transferred to nitrocellulose filters and blotted with appropriate antibodies. Twenty-five percent of the input is shown. (A) TFIIA. TFIIA was reconstituted with recombinant p12 and p55. The filter was probed with antibodies recognizing p55. (B) TBP (lanes 1, 3, and 5) and TFIIB (lanes 2, 4, and 6). The filter was probed with antisera recognizing recombinant TBP and TFIIB. (C) Pol II. The filter was probed with an anti-CTD mAb (8WG16).