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5-HT metabolism in hamster (A) and rat (B) liver mitochondrial preparations. 5-HT (10 μM) was incubated with freshly prepared hamster or rat liver mitochondria preparations (0.4 mg of mitochondrial protein per milliliter) in a 0.5-ml standard assay medium for 30 min. Concentrations of 5-HT and its metabolic products in the assay media then were analyzed by HPLC as described in Materials and Methods.
