Figure 1.

SDS-denaturing PAGE of PSII and other proteins. In A and B, samples were incubated with 4 mM [¹⁴C]methylamine-hydrochloride for 30 min. In A, the gel was stained with Coomassie brilliant blue. Samples not treated with [¹⁴C]methylamine give similar staining patterns to those shown here. In B, fluorography was performed. Lanes: 1, PSII-1; 2, PSII-2; 3, PSII-3; 4, MSP; 5, LHC; 6, PSI; AO, BSAO. In C, electrophoresis and fluorography were performed on PSII and amine oxidase. PSII-2 was incubated with 4 mM [¹⁴C]methylamine-hydrochloride for 30 min (lane 1), 60 min (lane 2), and 120 min (lane 3). Lane AO contains amine oxidase incubated with 4 mM [¹⁴C]methylamine-hydrochloride for 30 min. In D, electrophoresis and fluorography were performed on PSII-2 (lane 1), PSII-2 plus 5 molar equivalents of plastoquinone (lane 2), and purified plastoquinone (lane 3), after incubation of samples with 4 mM [¹⁴C]methylamine-hydrochloride for 30 min. Note that, unlike gels in A–C and E and F, this gel was run so that the dye front can be visualized. In E, no protein (lanes 1–3) or PSII-3 (lanes 4–6) was incubated with 2 mM [¹⁴C]formaldehyde (lanes 1 and 4), 4 mM [¹⁴C]methylamine-hydrochloride (lanes 2 and 5), or [¹⁴C]formaldehyde (2 mM) and [¹⁴C]methylamine-hydrochloride (4 mM) (lanes 3 and 6). The incubation time was 30 min. Lane AO contains amine oxidase incubated with 4 mM [¹⁴C]methylamine-hydrochloride for 15 min. In F, PSII-2 was incubated with 0.5 mM (lane 1), 1.0 mM (lane 2), 2.0 mM [¹⁴C]formaldehyde (lane 3), or 4 mM [¹⁴C]methylamine-hydrochloride (lane 4). The incubation time was 60 min. Lane AO contains amine oxidase incubated with 4 mM [¹⁴C]methylamine-hydrochloride for 15 min.