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High-affinity fluoride-promoted RhoA–p190 complex is aluminum-independent but requires magnesium. (A) Baculovirus p190 was incubated with GST-RhoA beads in the presence of NaF at the indicated concentrations and either the aluminum chelators deferoxamine (0.5 mM) or EGTA (5 mM) or AlCl3 (100 μM), as indicated. (B) The RhoA–p190 binding assay was performed in the presence or absence of EDTA (5 mM). p190 was detected by immunoblotting after SDS/PAGE.
