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. 1998 Mar 3;95(5):2222–2226. doi: 10.1073/pnas.95.5.2222

Figure 1.

Figure 1

Time course of receptor recycling. After AVP-promoted sequestration, the hormone was removed by acid wash and the sites on the cell surface were measured before and after incubation at 37°C. In all experiments the expression of the mutant receptors was determined by performing binding assays with a known number of intact cells. The number of receptors expressed by the different constructs as percent of wild type (WT) were 102 ± 10% for the V2–V1a chimera, 33 ± 5% for 345t, 86 ± 11% for 369t, 88 ± 5% for 362t, 102 ± 7% for S362A, 99 ± 12% for S363A, and 94 ± 8% for S362A V2R. (A) Recycling of WT (n = 16), V2–V1a chimera (n = 3), and 345t mutant (n = 6) receptors. (B) Recycling of WT (n = 16), 369t (n = 4), and 362t (n = 3) mutant receptors. (C) Recycling of WT (n = 16) and three mutant V2Rs carrying a single Ala substitution in the Ser cluster at positions 362 (n = 3), 363 (n = 3), or 364 (n = 3). Numbers in parentheses specify number of experiments performed.