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Schematic of the BMV RNA3 cDNA region in yeast centromeric plasmid pB3. The 3a and coat protein genes are indicated. The GAL1 promoter fused to the 5′ end of RNA3 cDNA allows gal-induced in vivo transcription of RNA3, and the hepatitis δ ribozyme cleaves the transcript at the natural 3′ end of RNA3. The bent arrow below the diagram shows the start of sequences for the subgenomic coat protein mRNA, RNA4, which is transcribed from the negative-strand RNA3 replication intermediate.
