Figure 4.

Nuclear run-on analysis of DNA-dependent RNA3 transcription. Yeast expressing the indicated BMV components were grown in gal-containing medium and permeabilized with sarkosyl, and nuclear RNA transcripts were labeled by incubating the permeabilized cells with [α-³²P]UTP for 10 min (14). Total RNA then was extracted and used to probe activated nylon membranes on which equal amounts of full-length, negative-strand, in vitro transcripts of RNA3 had been spotted and immobilized.