IL-1 receptor/IL-6 double knock out mice are not protected from titanium particle-induced osteolysis. Osteolysis was measured in the entire parietal bones (A) and in the suture regions (B). Data are presented as box plots showing the 10th, 25th, 50th, 75th, and 90th, percentiles. In A, n = 26–36 for all groups. In B, n = 13–18 for all groups. Panel C shows genotyping of the IL-1 receptor/IL-6 double knock out mice. PCR amplicons from three representative wild type and three representative double knock mice are shown. Tail clip DNA was amplified with primers specific for the IL-1 receptor gene, the IL-6 gene, and the neomycin cassette, respectively.