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. 1997 Jan 7;94(1):248–253. doi: 10.1073/pnas.94.1.248

Figure 5.

Figure 5

Effect of coexpressed SRC-1 on the transcriptional activity of WT and L454V mutant receptors. (a) 24-well plates of JEG-3 cells were transfected with 500 ng of MALTKLUC, 50 ng of WT or mutant vectors, 100 ng Bos–β-gal and varying amounts (0–100 ng) of SRC-1 expression vector, including empty vector where necessary to keep total vector DNA constant. Following incubation in the absence or presence of 100 nM T3, reporter activity was calculated and expressed as a percentage relative to the maximum corrected luciferase activity of WT receptor with 100 ng of SRC-1. Basal (open) versus stimulated (dark shaded) levels for WT and basal (light shaded) versus stimulated (solid) for L454V mutant receptor are shown. (b) JEG-3 cells were transfected with 500 ng of PALTKLUC reporter together with other plasmids as in a. Reporter activity with or without 100 nM T3 was calculated as above. Note that the scale of the vertical axes have been interrupted to show both basal and stimulated reporter activity.