Skip to main content
. 1998 Mar 3;95(5):2238–2243. doi: 10.1073/pnas.95.5.2238

Figure 2.

Figure 2

Characteristics of E. coli expressed RB47 protein. (A) Proteins from whole cell extract of untransformed E. coli strain (BL21), from BL21 cells transformed with the RB47 cDNA in a pET expression vector (RB47 cDNA), and from heparin agarose-purified proteins from C. reinhardtii, were detected with antisera against C. reinhardtii RB47 protein. (B) UV crosslinking of radiolabeled psbA RNA to E. coli expressed RB47 cDNA. Lane 1 contains the radiolabeled RNA only (no protein). Lane 2 contains whole cell extract from untransformed E. coli (BL21). Lane 3 contains heparin agarose purified proteins from E. coli transformed with the RB47 cDNA (RB47 cDNA), and lane 4 contains heparin agarose purified proteins from C. reinhardtii. Molecular mass is indicated at left.