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. 1997 Jan 7;94(1):271–276. doi: 10.1073/pnas.94.1.271

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Copyright © 1997, The National Academy of Sciences of the USA

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Genetic analysis of serogroup B301 (strains 1070 and 1069) and C301 (strains 1205, 1198, and 1204) N. meningitidis recovered from Oregon/Washington outbreak. (A) Nucleotide sequence alignment of the 3′ end of synC and downstream sequence in serogroup B strains NMB and 1070 (B-301#1), and serogroup C strains FAM18 and 1205 (C-301#1) [Pretty multiple sequence comparison program of the Genetics Computer Group sequence analysis package version 7.3.1 UNIX (33)]. The synC termination codon (TAA) and the synD/E start codons (ATG) are indicated in boldface type. (B) Nucleotide polymorphisms of the B301, C301, and other meningococcal strains within (i) a 909-bp PCR product containing the 5′ ends of both ctrA and synX and the 134-bp intergenic region separating these two genes (bp 1–319 are the 5′ end of ctrA, bp 320–453 are the 134-bp intergenic region, and bp 454–909 are the 5′ end of synX) (26), (ii) a 238-bp PCR product amplified from the 330-bp FKBP gene (28), and (iii) an 803-bp PCR product amplified from the 1128-bp recA gene (36). Regions were sequenced from strains 1070 (B301#1) (B), 1069 (B301#2) (B), FAM18 (C), 1205 (C301#1) (C), 1198 (C301#2) (C), 1204 (C301#3) (C), NMB (B), GA1002 (W-135), F8239 (A), GA0929 (Y), and GA1002 (W-135) and compared with the sequence of other neisserial strains (28, 36). The sequence of strain 1070 (B301#1) was used as the master sequence. Differences from the master sequence are indicated at the nucleotide positions within FKBP, recA, or the ctrA–synX PCR product (shown above), identity at a given position is indicated by a dash and deleted nucleotides are shown by dots.

Genetic analysis of serogroup B301 (strains 1070 and 1069) and C301 (strains 1205, 1198, and 1204) N. meningitidis recovered from Oregon/Washington outbreak. (A) Nucleotide sequence alignment of the 3′ end of synC and downstream sequence in serogroup B strains NMB and 1070 (B-301#1), and serogroup C strains FAM18 and 1205 (C-301#1) [Pretty multiple sequence comparison program of the Genetics Computer Group sequence analysis package version 7.3.1 UNIX (33)]. The synC termination codon (TAA) and the synD/E start codons (ATG) are indicated in boldface type. (B) Nucleotide polymorphisms of the B301, C301, and other meningococcal strains within (i) a 909-bp PCR product containing the 5′ ends of both ctrA and synX and the 134-bp intergenic region separating these two genes (bp 1–319 are the 5′ end of ctrA, bp 320–453 are the 134-bp intergenic region, and bp 454–909 are the 5′ end of synX) (26), (ii) a 238-bp PCR product amplified from the 330-bp FKBP gene (28), and (iii) an 803-bp PCR product amplified from the 1128-bp recA gene (36). Regions were sequenced from strains 1070 (B301#1) (B), 1069 (B301#2) (B), FAM18 (C), 1205 (C301#1) (C), 1198 (C301#2) (C), 1204 (C301#3) (C), NMB (B), GA1002 (W-135), F8239 (A), GA0929 (Y), and GA1002 (W-135) and compared with the sequence of other neisserial strains (28, 36). The sequence of strain 1070 (B301#1) was used as the master sequence. Differences from the master sequence are indicated at the nucleotide positions within FKBP, recA, or the ctrA–synX PCR product (shown above), identity at a given position is indicated by a dash and deleted nucleotides are shown by dots.