Table 4.

Significant expression differences of ID1, ID2, ID3 and ID4 proteins in the frontal cortex of Mecp2^−/y mice compared to the Mecp2^+/y mice at various developmental time points

		Mecp2+/y		Mecp2−/y
Brain Region	Age (days)	Mean ± SEM	Cell #	Mean ± SEM b	Cell #
Inhibitor of differentiation 1 - ID1 a
Frontal Cortex	E 15	4381 ± 59	848	5026 ± 96**	488
	P 7	4964 ± 19	2951	5639 ± 23***	2866
	P 28	4479 ± 28	1943	5015 ± 39**	1016
	P 49	4441 ± 25	2581	5112 ± 43***	1070
	P 56	3750 ± 20	2054	3876 ± 21	1918
	P 70	4643 ± 43	1454	5097 ± 37**	2046
Inhibitor of differentiation 2 - ID2 a
Frontal Cortex	E 15	4432 ± 51	859	5078 ± 65**	519
	P 7	5100 ± 32	1090	6152 ± 44***	1076
	P 28	4387 ± 44	2122	4622 ± 48**	947
	P 49	3457 ± 25	1189	4228± 58***	523
	P 56	3282 ± 18	1064	3552 ± 26**	990
	P 70	4397 ± 52	699	5665 ± 63***	1002
Inhibitor of differentiation 3 - ID3 a
Frontal Cortex	E 15	4052 ± 48	1130	3917 ± 62	481
	P 7	4577 ± 38	2464	5211 ± 38***	2032
	P 28	6437 ± 74	627	6494 ± 60	532
	P 49	6386 ± 82	561	6687 ± 86	376
	P 56	5034 ± 28	1122	5390 ± 40*	699
	P 70	6295 ± 70	581	6773 ± 64*	737
Inhibitor of differentiation 4 - ID4 a
Frontal Cortex	E 15	6568 ± 84	817	6295 ± 123	450
	P 7	5151± 45	566	5347 ± 43	650
	P 28	4095 ± 38	1062	4784 ± 44*	516
	P 49	3653 ± 34	605	4806 ± 41**	602
	P 56	3645 ± 36	436	4148 ± 35**	579
	P 70	4607 ± 60	438	5722 ± 55***	557

^a- ID1, ID2, ID3, ID4 rabbit polyclonal antibodies obtained from commercially available source (Santa Cruz Biotechnologies, Inc).

^b- Significance determined by chi-square and P values denoted by asterix.

^*P ≤ 0.05,

^**P ≤ 0.005,

^***P ≤ 0.0005.