Table 3.
Treatment | Duration | Percentage mER positive cells |
---|---|---|
Vehicle control | 3 min | 93.3 ± 9.8 |
1 nME2 | 3 min | 8.4 ± 10.6* |
1 nME2 | 15 min | 89.7 ± 8.7 |
10 nM DES | 3 min | 5.1 ± 6.3* |
10 nM DES | 15 min | 91.2 ± 5.4 |
10 nM NP | 3 min | 7.3 ± 4.5* |
10 nM NP | 15 min | 90.9 ± 5.2 |
Cells grown on coverslips and serum withdrawn were then treated with vehicle (0.01 % ethanol), E2, DES, or nonylphenol (NP) for the times and at the concentrations indicated. Fixed and stained cells for each condition were examined by microscopy; multiple fields were examined and 200 cells for each condition were scored for the presence of mER antigen (mER positive).
Significant difference from vehicle control at the P < 0.001 level. Vehicle treatment at 15 min and a group which had no treatment were not statistically different from the 3 min vehicle treatment results (data not shown).