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. 2007 Jul 3;7:65. doi: 10.1186/1471-2180-7-65

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Copyright © 2007 Bullard et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blot analysis of recombinant E. coli cells treated with proteinase K. E. coli carrying the plasmids pELO35.Hag (lanes 1 and 2), pBBHS2.24 (lanes 3 and 4) and pBBHS3.20 (lanes 5 and 6) were incubated for 15 min on ice in the presence (lanes 2, 4 and 6) or absence (lanes 1, 3 and 5) of proteinase K. These cells were lysed, resolved by SDS-PAGE, transferred to PVDF membranes and probed with the anti-Hag antibody 5D2 (panel A) or anti-TonB antibody 4F1 (panel B). Molecular weight markers are shown to the left in kDa.