Table 1.
Strains and plasmids
| Strain or plasmid | Description | Source or reference |
| M. catarrhalis | ||
| [26] | ||
| O35E | Wild-type isolate | [26] |
| O35E.TN2 | Isogenic hag mutant strain of O35E | [26] |
| O12E | Wild-type isolate | [26] |
| O12E.Hag | Isogenic hag mutant strain of O12E | [26] |
| TTA37 | Wild-type isolate | [26] |
| TTA37.Hag | Isogenic hag mutant strain of TTA37 | [26] |
| V1171 | Wild-type isolate | [26] |
| V1171.Hag | Isogenic hag mutant strain of V1171 | [26] |
| McGHS1 | Wild-type isolate | [26] |
| McGHS1.Hag | Isogenic hag mutant strain of McGHS1 | [26] |
| E. coli | ||
| EPI300 | Cloning strain | Epicentre |
| TUNER | Expression strain | Novagen |
| Plasmids | ||
| pETcoco-1 | Protein expression vector, Cmr | Novagen |
| pBBCT.77 | pETcoco-1 expressing O35E-Hag aa 1358–1964 joined to six N-terminal histidine residues, Cmr | This study |
| pCC1.3 | Adherence negative plasmid control | [48] |
| pCC1 | Cloning vector, Cmr | Epicentre |
| pELO35.Hag | pCC1 expressing the entire O35E hag gene product, Cmr | [26] |
| pBBO12.Hag | pCC1 expressing the entire O12E hag gene product, Cmr | [26] |
| pSV1171.Hag | pCC1 expressing the entire V1171 hag gene product, Cmr | [26] |
| pBBHS2.24 | Deletion derivative of pELO35.Hag, missing aa 71–283 | This study |
| pBBHS3.20 | Deletion derivative of pELO35.Hag, missing aa 71–384 | This study |
| pBBHS8.18 | Deletion derivative of pELO35.Hag, missing aa 71–384 and 1546–1707 | This study |
| pBBHS6.22 | Deletion derivative of pELO35.Hag, missing aa 436–1538 | This study |
| pBBHS5.12 | Deletion derivative of pELO35.Hag, missing aa 71–283 and 919–1538 | This study |
| pBBHS10.9 | Deletion derivative of pELO35.Hag, missing aa 71–384, 746–1193 and 1546–1707 | This study |
| pBBHS10.32 | Deletion derivative of pELO35.Hag, missing aa 71–705 and 1546–1707 | This study |