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Immunohistochemical detection of Cx43 of maturation arrest (Johnsen score 3.24). Positive area for Cx43 on the plasma membrane of Sertoli cells was decreased and restricted to the basal compartment of seminiferous tubules while strong signal of Cx43 was found on the plasma membrane of Leydig cells. (A) H & E staining, ×200. (B) ×200. (C) ×400. (D) Negative control, ×200. Bar=50 µm.
