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Relationship among the scattering densities of the bulk solvent, protein, and protein–solvent interface for x-rays and neutrons in H2O and D2O. (1) Bulk solvent, (2) a shell with density 20% above that of the bulk solvent, (3) mobility of the side chains on the protein surface; scattering density in the interface is drawn in the middle between those of protein and of bulk; (4) protein. Scattering density of protein in D2O is larger than that in H2O because of H/D exchange.
