Figure 3.—

puc is required in border cells with low PVR signaling. (A) Stage 10 egg chambers, stained with DAPI (blue) to show nuclei, anti-β-galactosidase antibody (green) to reveal mutant cells, and phalloidin (red) from females of the following genotypes: hsFLP,UAS-lacZ/+; slbo-Gal4,UAS-dnPVR/+; FRT80,FRT82/FRT82,tub-Gal80 (top) and hsFLP,UAS-lacZ/+; slbo-Gal4,UAS-dnPVR/+; FRT80,FRT82, puc^PBac3929/FRT82,tubGal80 (bottom). Bar, 20 μm. (B–F) Quantification of border cell migration toward the oocyte (posterior migration) in stage 10 egg chambers. (B) puc^PBac3929 clones with or without dnPVR and control MdnP clones from females of the genotypes hsFLP,UAS-lacZ/+; slbo-Gal4,UAS-dnPVR/+; FRT80,FRT82/FRT82,tub-Gal80 (left, n = 10); hsFLP,UAS-lacZ/+; slbo-Gal4,UAS-dnPVR/+; FRT80,FRT82, puc^PBac3929/FRT82,tub-Gal80 (middle, n = 21); and hsFLP,UAS-GFP/+; tub-Gal4/+; FRT80,FRT82, puc^PBac3929/FRT82,tub-Gal80 (right, n = 10). (C) Control border cells overexpressing Pvf1 (left; genotype hsFLP,UAS-GFP/EP-Pvf1; tubGal4/+; FRT80,FRT82/FRT82,tubGal80, n = 19) and puc^PBac3929 clones overexpressing Pvf1 (right; genotype hsFLP,UAS-GFP/EP-Pvf1; tubGal4/+; FRT80,FRT82,puc^PBac3929/FRT82,tubGal80; n = 20). (D) Border cell clusters expressing dnPVR, hep^ACT, or both (n = 93, 272, 80). (E) Clusters expressing hep^ACT in control, Pvf1/+, or Pvf1 mutant background (n = 42, 149, 22). (F) Clusters expressing uniform Pvf1, alone or with hep^ACT (n = 203, 73). Genotypes are indicated and include the controls in each experiment. (G) Quantification of dorsal migration in puc^PBac3929 border cell clones (from hsFLP/+; FRT80,FRT82, puc^PBac3929/FRT82,UbiGFP females, n = 23), border cells expressing hep^ACT (slbo-Gal4/+; UAS- hep^ACT/+, n = 20), and the control (slbo-gal4/+, n = 12). (H) Follicular epithelium of stage 10 egg chamber from female of the genotype hsFLP/+; kek-lacZ/+; FRT80,FRT82, puc^PBac3929/FRT82,UbiGFP, stained with anti-β-galactosidase antibody (β-gal, blue in overlay) to reveal kek-lacZ reporter expression and phalloidin (red in overlay). puc^PBac3929mutant cells are marked by absence of GFP (green in the overlay). Dorsal is to the top. Bar, 5 μm.