Figure 1.—

C. elegans exposed to E. faecalis produces more hydrogen peroxide than non-pathogen-exposed worms and this production is inhibited by DPI. (A) Hydrogen peroxide production from 100 nematodes/well after exposure to B. subtilis, E. coli, E. faecalis, or E. faecalis (menB) with and without DPI. (B) Absorbance measurements of the wells in A with the two samples of each condition averaged and the no-worm controls subtracted out. The amount of hydrogen peroxide produced per minute was calculated by comparison to a standard curve (data not shown). The error bars correspond to the standard deviation. The difference between the amount of hydrogen peroxide produced with and without DPI was significant (P < 0.05) for the E. faecalis strains as indicated by the asterisks. This experiment was repeated three times with similar results.