TABLE 1.
Marker segregation in F2 lines
| F2 linesc
|
|||||
|---|---|---|---|---|---|
| Markera | Primersb | Fragment size (bp) | % positive | P (1:1) | P (3:1) |
| H1 | See text. | 359 | 55.5 | 0.272 | 6.65E-06 |
| H2 | See text. | 250 | 48.6 | 0.782 | 1.11E-09 |
| AF1a | E+AT/M+GA | 258 | 49.2 | 0.867 | 2.42E-09 |
| AF1b | E+AT/M+GA | 243 | 50.8 | 0.867 | 2.41E-08 |
| AF2 | E+AT/M+GG | 231 | 42.5 | 0.136 | 6.58E-14 |
| AF3a | E+TA/M+GG | 233 | 54.2 | 0.402 | 1.54E-06 |
| AF3b | E+TA/M+GG | 227 | 45.8 | 0.402 | 1.57E-11 |
| AF4 | E+TA/M+GG | 256 | 50.8 | 0.867 | 2.41E-08 |
| AF5a | E+CG/M+AG | 257 | 40.0 | 0.046 | 6.32E-16 |
| AF5b | E+CG/M+AG | 184 | 60.0 | 0.046 | 5.32E-04 |
| AF6 | E+CG/M+AG | 218 | 57.7 | 0.124 | 6.41E-05 |
| AF7 | E+AT/M+GA | 190 | 53.0 | 0.548 | 3.79E-07 |
| AF8 | E+AT/M+GG | 100 | 48.6 | 0.782 | 1.11E-09 |
| AF9 | E+AT/M+GG | 180 | 43.1 | 0.167 | 1.73E-13 |
| AF10 | E+CG/M+AG | 90 | 47.3 | 0.583 | 1.47E-10 |
| AF11 | E+ACC/M+G | 280 | 47.2 | 0.579 | 1.41E-10 |
| AF12 | E+ACC/M+G | 230 | 49.4 | 0.912 | 3.59E-09 |
| AF22a | E+CAA/M+TA | 205 | 47.2 | 0.579 | 1.41E-10 |
| AF22b | E+CAA/M+TA | 220 | 52.8 | 0.579 | 2.87E-07 |
| AF23a | E+ACG/M+CTT | 93 | 47.8 | 0.657 | 3.24E-10 |
| AF23b | E+ACG/M+CTT | 79 | 52.2 | 0.657 | 1.44E-07 |
| AF24a | E+CG/M+CTT | 93 | 50.3 | 0.955 | 1.14E-08 |
| AF24b | E+CG/M+CTT | 79 | 49.7 | 0.955 | 5.28E-09 |
| AF25a | E+CA/M+GA | 154 | 47.0 | 0.543 | 9.47E-11 |
| AF25b | E+CA/M+GA | 155 | 53.0 | 0.543 | 3.94E-07 |
a
Markers labeled “a” and “b” after the same numerical designation are allelic.
b
E and M stand for universal AFLP primers (E: GACTGCGTACCAATTC; M: GATGAGTCCTGAGTAA). Sequences of H1 and H2 primers are listed in materials and methods.
c
P represents the P-value of the chi-squared goodness-of-fit test for 1:1 or 3:1 segregation.