Figure 6.—

Nuclear Sic1-wt-GFP is detected only during M/G₁; Sic1-0P-GFP is nuclear throughout the cell cycle. Cells containing SIC1-wt-GFP or SIC1-0P-GFP, expressed from the endogenous locus were analyzed by time-lapse fluorescence microscopy, with illumination every 3 min. Selected frames from movies are shown to illustrate Sic1-wt-GFP appearing as a nuclear signal in large-budded cells (top, green arrows) and then disappearing rapidly from the mother-cell nucleus and more slowly from the daughter-cell nucleus (top, red arrows). Sic1-wt-GFP disappearance occurs shortly before bud emergence (top, blue arrows). Sic1-0P-GFP was nuclear throughout the cell cycle, including before and after bud emergence (bottom, blue arrows) and before and after nuclear division (pink arrows). The disappearance of Sic1-0P-GFP from nuclei was never observed. In both series, for clarity, only some events are labeled with arrows.