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Ure2 protein and transcript levels in various media. Strains were grown in the following media: YPAD, proline medium (PRO), PRO supplemented with 50 mm ammonium sulfate (NH4) or 2 g/liter amino acids (AA). (A) Western blot analysis of relative Ure2p levels. (B) Northern blot analysis of relative URE2 transcript levels. (C) Levels of truncated Ure2pΔ1–80, but not full-length Ure2p, are increased in response to supplemental ammonium (0–50 mm 
from left to right) in proline medium.
