Figure 8.—
Integrated SUP35 gene truncations function completely in translational readthrough assay. (A) Nonsense suppression was assayed using a dual luciferase reporter with the different stop codons and using full-length Sup35p or a truncation lacking the prion domain. (B) Strains coding Sup35p with and without the prion domain were plated to selective media that were previously reported to reveal phenotypic differences for strains lacking the prion and middle domain of Sup35p. Top row, SUP35; bottom row, SUP35MC, 10-fold dilutions.