Table 1. Constrained elements at the Agrp locus identified by GERP.

RS scorea	Start Positionb	End Positionc	Lengthd	Overlapping Region
234.0	+563	+710	147 bp	Exon 4
67.1	−832	−792	40 bp	IRE A
64.2	−8	+46	54 bp	Exon2
61.4	−472	−428	44 bp	IRE B
32.1	−755	−731	24 bp	STATA
26.8	−179	−167	13 bp	TATA
26.2	−527	−515	12 bp	STAT B
21.8	+306	+335	29 bp	Exon 3
17.6	+546	+560	14 bp	Exon 4
16.3	−363	−348	15 bp	None
13.9	+283	+297	14 bp	Exon 3

^aThe Rejected Substitution score is a measurement of evolutionary constraint, based on Genomic Evolutionary Rate Profiling [18]. The table includes a list of scores above the maximum false positive score for this data set (see methods).

^b,cThe initial and terminal columns within the sequence alignment defining the region of evolutionary constraint (coordinates based on mouse genomic sequence, relative to the translational start site of Agrp).

^dLength of evolutionarily constrained region defined as the sum of consecutive alignment columns with positive RS scores.