Table 1.
Composition of solutions
| Solution | K-propionate | Imidazole | EGTA | ATP | MgCl2 | CaCl2 | Glutathione | DM-nitrophen |
|---|---|---|---|---|---|---|---|---|
| Relaxing | 80 | 20 | 10 | 4 | 5 | — | — | — |
| Contracting* | 80 | 20 | 10 | 4 | 5 | 10.1 | — | — |
| Prephotolysis | 80 | 20 | 0.1 | 0.3 | 1.3 | — | — | — |
| Photolysis | 87 | 22 | — | 0.22 | 4.3 | 0.2 | 11 | 2.6 |
All concentrations in millimoles per liter. Overall ionic strength of the solutions was 170 mM, and their pH was adjusted to 7.2 by KOH. DM-nitrophen, 1-(2-nitro-4,5-dimethoxyphenyl)-N,N,N′,N′-tetrakis [(oxycarbonyl)methyl]-1,2-ethanediamine.
*
pCA 4.5.