Figure 5.

Dose-response curve for IHPS inhibition of the evoked and spontaneous CA release from DG-permeabilized cells during the initial phase. The cells were permeabilized with DG buffer for 4 min, incubated two times for 2 min with the buffer without DG, and then stimulated with Ca²⁺ (0 or 10 μM) buffer for 1.5 min. IHPS (IP₄, IP₅, or IP₆) was added during the incubation for the last 2 min and the subsequent stimulation. The amounts of released CA are expressed as the percentage of total cellular content. Data are the mean ± SEM of at least eight experiments.