Figure 4.
Inhibition of the MAP1- and MAP2-promoted microtubule assembly by AD P-tau. Polymerization of tubulin was determined as described. The assembly reaction was carried out using (i) 1.0 mg/ml MAP1 or a mixture of MAP1 and 2 mg/ml AD P-tau (a) or (ii) 1.0 mg/ml MAP2 or MAP2 plus 2 mg/ml AD P-tau (b). For comparison, the inhibition of tau-promoted microtubule assembly is reproduced (c) from our previous report (5).