Figure 5.
Effect of protein synthesis inhibition on AA-NAT mRNA levels. Dissociated pineal cells from 1-day-old chicks were cultured for 5 days in LR 12:12 (white light on at ZT 0) and were then transferred to constant red. At different times of the subjective cycle (zeitgeber times are indicated as a reference) cells were incubated with 10 μM cycloheximide (CHX) for 6 hr. Total RNA was prepared from two groups of ≈6 × 106 cells and analyzed by Northern blot (20 μg total RNA per lane). AA-NAT mRNA values have been normalized to the GAPDH signal. All values represent the mean of duplicate determinations and are expressed relative to the ZT 5 control in RR.