DNA ligases of mycobacteria, E. coli, and bacteriophage T4. A schematic diagram of conserved domains within the various DNA ligases used in this study is shown. The number of amino acids in each specific protein is indicated. Approximate relative positions of domains conserved within more than one of the proteins are shown by the shaded regions. Domains were identified from their reference within the Pfam (8) or SMART (15) database, as indicated. (A) NAD+-dependent ligases from M. smegmatis (MsLigA), M. tuberculosis (MtLigA), and E. coli (EcLigA). (B) ATP-dependent DNA ligases from M. smegmatis (MsLigB, MsLigC1, MsLigC2, and MsLigD) and bacteriophage T4 (T4Dnl).