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PRP1 confers pentamidine resistance in Leishmania infantum (Li) axenic amastigotes. A growth curve is shown for L. infantum axenic amastigotes in MAA/20 medium (24) in the presence of pentamidine. Promastigote parasites were transfected with the plasmids indicated (the control plasmid, pSNBR [22]; PRP1 [pSNBR/8kb SmaI-A] [3]; and PRP1-GFP [4]) and induced for differentiation to axenic amastigotes (24). Experiments were done in triplicate.
