FIG. 3.

Site-specific footprints of chain-terminated complexes. (A) RT complexes with radiolabeled template and chain-terminated primers were treated with KOONO as described in Materials and Methods. The next templated nucleotide (dCTP) was added at increasing concentrations prior to KOONO cleavage. The sequence underneath the gels shows the positions of site-specific cleavage with KOONO treatment. Cleavage at position −8 is indicative of the pretranslocation complex, and cleavage at position −7 is indicative of the posttranslocation complex. (B) Graphical representation of data shown in panel A.