TABLE 2.
Effects of inhibitor and enzyme on the translocation status of RT
| Chain terminator | Posttranslocated state | % or concna
|
|
|---|---|---|---|
| WT RT | 4Y RT | ||
| ddAMP | No dCTP | 65% | 54% |
| IC50(dCTP) | ND | ND | |
| IC80(dCTP) | 0.2 μM | 0.3 μM | |
| TFV | No dCTP | 45% | 38% |
| IC50(dCTP) | 0.1 μM | 0.3 μM | |
| IC80(dCTP) | 3 μM | 2 μM | |
| AZA-MP | No dCTP | 14% | 1% |
| IC50(dCTP) | 10 μM | 27 μM | |
| IC80(dCTP) | 100 μM | >100 μM | |
| AZT-MP | No dCTP | 6% | 0% |
| IC50(dCTP) | 8 μM | 8 μM | |
| IC80(dCTP) | 31 μM | 50 μM | |
a
Percentage of complexes that existed posttranslocationally in the absence of nucleotide substrate or nucleotide concentration required to form 50% or 80% of the complex population in its posttranslocation state. Percentages are means for at least three independent experiments. The standard deviation was below 10%. ND, not determined because the posttranslocated complex was >50% in the absence of dCTP.