FIG. 3.
IMGT “collier de perle” graphical 2-dimensional representation of 2LF. (A) Fd fragment; (B) light chain. IMGT “collier de perle” representations are displayed according to IMGT unique numbering (29, 30). Dots indicate differences between 2LF and the human genes most similar to 2LF. Hydrophobic amino acids (those with positive hydropathy index values, i.e., I, V, L, F, C, M, and A) and tryptophan (W) are shown in blue circles. All proline (P) residues are shown in yellow circles. The complementarity-determining region (CDR-IMGT) sequences are delimited by amino acids shown in squares (anchor positions), which belong to the neighboring FR (FR-IMGT). Gray circles correspond to missing positions according to IMGT unique numbering. Colors of circle outlines indicate regions: in the VH domain, red for CDR1-IMGT, orange for CDR2-IMGT, and purple for CDR3-IMGT; in the Vκ domain, blue for CDR1-IMGT, green for CDR2-IMGT, and turquoise for CDR3-IMGT.