TABLE 3.

Localization and evaluation of differences between 2LF framework regions and those encoded by human germ line genes most similar to 2LF^a

Region	No. (%) of residues with the following score:
	Identical	Very similar	Similar	Dissimilar	Very dissimilar
H-FR1	21	0	2	2	1
H-FR2	15	0	1	1	0
H-FR3	32	0	2	5	0
H-FR4	8	0	1	1	1
Total H-FR	76 (81.7)	0 (0)	6 (6.4)	9 (9.8)	2 (2.1)
L-FR1	23	1	1	1	0
L-FR2	14	1	0	2	0
L-FR3	34	0	1	2	2
L-FR4	8	0	1	0	0
Total L-FR	79 (86.8)	2 (2.2)	3 (3.3)	5 (5.5)	2 (2.2)
Total FR	155 (84.2)	2 (1.1)	9 (4.9)	14 (7.7)	4 (2.2)

^aDifferences between 2LF framework amino acids and those coded by the most similar human genes are evaluated on a 5-level scale, from identical to very dissimilar (see “Computational analysis” under Results), and located in each framework region.