FIG. 5.

Kinetics of excision/rescue of purine and pyrimidine analogues. (A) The velocity of the combined excision/rescue reaction was analyzed with template T20-C16 and 3′-dCMP-, 2′-O-Me-CMP-, and 2′-C-Me-CMP-terminated primers (lanes 0). The reaction was monitored in a time course experiment for 1, 2, 5, 10, 20, 30, and 45 min (lanes 1 to 7, respectively). The percentage of rescued primer (position +17) was quantified relative to the amount of total RNA synthesis products. (B) The same experiment was performed with 3′-dAMP, 2′-O-Me-AMP, and 2′-C-Me-AMP, using the T20-A16 RNA template. Rates of the reactions are reported in Table 1.