TABLE 1.
Sequences of primers used for detection of blaCTX-M genes and their genetic environment
| Primer name | Primer sequence | Location |
|---|---|---|
| CTX-MA1 | 5′-SCSATGTGCAGYACCAGTAA-3′ | blaCTX-M gene |
| CTX-MA2 | 5′-CCG CRA TAT GRT TGG TGG TG-3′ | blaCTX-M gene, reverse primer |
| CTXM2A | 5′-GCC GCT CAA TGT TAA CGG-3′ | blaCTX-M gene (CTX-M-2 group) |
| CTXM2B | 5′-GAA ACC GTG GGT TAC GAT-3′ | blaCTX-M gene (CTX-M-2 group), reverse primer |
| CTXM9A | 5′-CTG ATG TAA CAC GGA TTG AC-3′ | blaCTX-M gene (CTX-M-9 group) |
| CTXM9C rev | 5′-AGC GCC CCA TTA TTG AGA G-3′ | blaCTX-M gene (CTX-M-9 group), reverse primer |
| TOHO2b rev | 5′-TTA CAG CCC TTC GGC GAT-3′ | blaCTX-M gene (CTX-M-9 group), reverse primer |
| CTXMpréB | 5′-CAC TTT GCC GTC GTC TAA GGC G-3′ | blaCTX-M gene (CTX-M-1 group), reverse primer |
| ISEcpPROM+ | 5′-TGC TCT GTG GAT AAC TTG C-3′ | ISEcp1 upstream of the promoter |
| ISEcpPROM− | 5′-GCA GTC TAA ATT CTT CGT G-3′ | ISEcp1 downstream of the promoter |
| IS903Bint | 5′-GCT TTT TGA CTT TCC ACT CGC-3′ | IS903B transposase, reverse primer |
| Orf513-D3 | 5′-CTC ACG CCC TGG CAA GGT TT-3′ | Orf513 |
| Orf513-D5 | 5′-CTT TTG CCC TAG CTG CGG T-3′ | Orf513, reverse primer |
| Orf513-5′ext | 5′-CAG CTG GTA GAG CAG CGT C-3′ | 5′ end of Orf513, reverse primer |
| OS5 | 5′-TTA TCT CCC TGT TAG CCA CC-3′ | blaSHV gene |
| OS6 | 5′-GAT TTG CTG ATT TCG CCG G-3′ | blaSHV gene, reverse primer |
| PRETEM-1 | 5′-GTA TCC GCT CAT GAG ACA ATA-3′ | blaTEM gene |
| PRETEM-2 | 5′-TCT AAA GTA TAT ATG AGT AAA CTT GGT CTG-3′ | blaTEM gene, reverse primer |