TABLE 1.
Strains and plasmids
| B. subtilis strain or plasmid | Relevant genotype/characteristics | Source or reference |
|---|---|---|
| Strains | ||
| 168-7G5 | Derivative of B. subtilis 168; surfactin positive | 46 |
| 168-8G5 | Derivative of B. subtilis 168; surfactin negative | 2 |
| AG665 | Cmr Δspo0H | 19 |
| ATCC6633 | Mycosubtilin positive; surfactin positive | 9 |
| BD1777 | Cmr ΔcomA | 12 |
| BV12E12 | (ATCC 6633) KmrmycA-lacZ; mycosubtilin negative | This work |
| BV12E13 | (ATCC 6633) KmrsrfAD-lacZ | This work |
| BV12E14 | (ATCC 6633) Kmrmyc-lacZ; mycosubtilin positive | This work |
| BV12E15 | (168-8G5) KmrsrfAD-lacZ; surfactin negative | This work |
| BV12E16 | (ATCC 6633) Cmr KmrmycA-lacZ ΔcomA | This work |
| BV12E18 | (ATCC 6633) Cmr KmrmycA-lacZ ΔabrB | This work |
| BV12E20 | (ATCC 6633) Cmr KmrmycA-lacZ ΔsinR | This work |
| BV12E22 | (ATCC 6633) Spr KmrmycA-lacZ ΔcssS | This work |
| BV12E24 | (ATCC 6633) Cmr KmrmycA-lacZ ΔdegU | This work |
| BV12E25 | (ATCC 6633) Cmr KmrmycA-lacZ ΔsrfAA | This work |
| BV12E27 | (168-8G5) Kmrmycp-lacZ; surfactin negative | This work |
| BV12E28 | (168-7G5) Kmrmycp-lacZ; surfactin positive | This work |
| BV12E29 | (ATCC 6633) Cmr KmrmycA-lacZ Δspo0K | This work |
| BV12E31 | (ATCC 6633) Cmr KmrmycA-lacZ Δspo0H | This work |
| BV12E32 | (ATCC 6633) Cmr KmrsrfAD-lacZ Δspo0K | This work |
| BV12E33 | (ATCC 6633) Cmr KmrsrfAD-lacZ Δspo0H | This work |
| BV12E35 | (168-8G5) Kmrmycp-lacZ ΔcomA; surfactin negative | This work |
| BV12E39 | (168-8G5) Kmrmycp-lacZ ΔabrB; surfactin negative | This work |
| BV12E40 | (168-7G5) KmrsrfAD-lacZ; surfactin positive | This work |
| BV12I11 | (ATCC 6633) Cmr KmrsrfAD-lacZ ΔcomA | This work |
| BV12I37 | (ATCC 6633) Cmr KmrmycA-lacZ ΔcodY | This work |
| BV12I38 | (ATCC 6633) Cmr KmrsrfAD-lacZ ΔcodY | This work |
| BV15D29 | Spr Kmr ΔcssS | 18 |
| IS432 | Cmr ΔsinR | 10 |
| JH12586 | Cmr ΔabrB | 31 |
| KI566 | Cmr ΔSpo0K | 37 |
| Plasmids | ||
| pGSP12 | Emr; contains comK | 47 |
| pLGW300 | Kmr; contains promoterless spo0V-lacZ fusion | 49 |
| pUC19C | Ampr Cmr | Lab collection, unpublished |