FIG. 4.
Detection of Mesorhizobium sp. signal molecules by the AHL monitor strain P. putida F117(pKR-C12). The biosensor was grown in the presence of 3-oxo-C12-HSL or compound 1 or 2 in a concentration range of 0 to 1,000 nM. Green fluorescence of the cultures was recorded with a microtiter plate reader at 515 nm. The measured values were corrected for autofluorescence and plotted as a function of AHL concentration. Fluorescence of the sensor in the presence of 1,000 nM 3-oxo-C12-HSL was arbitrarily set to 100%.