FIG. 5.
Measurements of protease activity (A) and pyoverdin production (B) in wild-type P. aeruginosa PAO1 and the AHL-deficient lasI rhlI double mutant grown in the absence or presence of 350 nM of 3-oxo-C12-HSL and 700 nM of compound 1 or 2. Sterile filtered supernatants were used for enzymatic measurements of proteolytic activities on azocasein and spectrophotometric quantification of pyoverdin. The activity of the wild type was arbitrarily set to 100%. The data represent mean values from three independent experiments. Error bars represent the standard errors of the means.