TABLE 2.
Predicted C. hutchinsonii glycohydrolases that may be involved in hemicellulose digestion
| Gene | Predicted function of gene producta | Predicted localizationb | Mol massc (kDa) | Modular structured |
|---|---|---|---|---|
| CHU_1155 | Candidate xyloglucanase | Extracellular | 132.2 | GH74-PKD-PKD-PKD-PKD-PKD-D5 |
| CHU_1239 | Candidate acetylxylan esterase/xylanase | Extracellular/outer membrane | 148.1 | CE6-X1-GH10-X1-CBM4-PKD-PKD-PKD-PKD-D5 |
| CHU_1240 | Candidate xylanase/esterase | Extracellular/outer membrane | 183.0 | CE4-GH8-CBM9-PKD-PKD-PKD-PKD-PKD-PKD-X1-D5 |
| CHU_2041 | Candidate acetylxylan esterase/β-xylosidase/α-l-arabinofuranosidase | Extracellular/outer membrane | 166.6 | CE6-GH43-X1-X1-CBM9-PKD-PKD-PKD-PKD-X1-D5 |
| CHU_2042 | Candidate xylanase | Extracellular/outer membrane | 127.4 | GH5-X1-CBM9-PKD-PKD-PKD-PKD-X1-D5 |
| CHU_2043 | Candidate xylanase | Extracellular/outer membrane | 128.5 | GH10-X1-CBM9-PKD-PKD-PKD-PKD-PKD-X1-D5 |
| CHU_2044 | Related to β-xylosidases/α-l-arabinofuranosidases | Extracellular/outer membrane | 154.4 | GH43-CBM6-X1-CBM9-PKD-PKD-PKD-X1-D5 |
| CHU_2105 | Candidate xylanase | Unknown | 76.3 | CBM4-GH10 |
| CHU_2379 | Candidate xylanase | Extracellular/outer membrane | 119.5 | GH11-CBM9-PKD-PKD-PKD-PKD-PKD-X1-D5 |
| CHU_0353 | Candidate β-mannanase | Extracellular | 86.6 | CBM6-GH26-PKD-PKD-D5 |
Assigned by routines used for updating the CAZY database (http://www.cazy.org/CAZY/), using the criteria indicated in Table 1.
Predicted using the default settings of PSORTb (21). Predicted lipoproteins were identified using LipoP (33).
Molecular mass of primary product of translation, including any predicted signal peptide.
Modular structure is indicated by the following abbreviations: CE, carbohydrate esterase (number indicates family), as assigned by CAZY; CBM, carbohydrate binding module (number indicates family), as assigned by CAZY; D5, carboxy-terminal domain of Rhodothermus marinus xylanases predicted to be involved in attachment to the cell surface (34); GH, glycoside hydrolase (number indicates family), as assigned by CAZY; PKD, polycystic kidney disease protein PKD1 (Pfam number PF00801); and X1, conserved domain of unknown function.