Validation of the DNA oligonucleotide array for identifying pathogens in food. (A) Genomic DNA from bacterial isolates recovered from a 24-h enrichment broth was labeled with CyDye and hybridized to the microarray. The average fluorescence signal for each C. jejuni and C. coli probe is color coded, as described in the legend to Fig. 1. (B) Morphology of individual cells as assessed by phase-contrast microscopy at a magnification of ×1,000. (C) Multiplex PCR assay (28) performed to corroborate the microarray data for species identification of Campylobacter isolates from retail chicken samples. Lanes M, 100-bp DNA ladder; lane 1, C. coli strain RM1166; lane 2, C. jejuni strain RM1221; lane 3, isolate BQ25; lane 4, isolate BQ26; lane 5, isolate BQ44; lane 6, isolate BQ41.