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Regression coefficient (r2), amplification efficiency (E), and slope of four standard curves for real-time PCR amplification of rRNA genesa
| Sample or isolate analyzed | r2 value | E value | Slope |
|---|---|---|---|
| Bacteria | |||
| VA 6 | 0.99 | 69.3 | 4.372 |
| EK13/8 | 0.99 | 70.4 | 4.321 |
| Flavobacteria | |||
| VA 6 | 0.98 | 78.8 | 4.142 |
| EK13/8 | 0.99 | 71.6 | 4.262 |
Either primer pair 27f and 519r (Bacteria) or pair 27f and 588r (Flavobacteria) was used. Standard curves for the environmental sample (EK13/8) and isolate VA 6 (EF078979) are shown.
