TABLE 2.
Sequence tag | Recombinant plasmid | Insert size (bp) | Associated gene(s)a | Protein homolog(s) (accession no.) | Domain organizationb | Protein classification | Signature motif(s) or substrate specificity | Predicted substrate specificity | Necessary for FK228 biosynthesis |
---|---|---|---|---|---|---|---|---|---|
P3-A6-SP6 | pP3-A6 | 2,826 | NNc | JamL (AAS98783) | KS-ATi | Type I PKS | QTRTAQ, GHSYG, and AAFH in AT domain | MCoA | No |
P4-B4-SP6 | pP4-B4 | 3,612 | depD | BmyB (CAE11249) | Ci-A-PCP-E | Type A NRPS | DLFEMSLIWK in A domain | l-Cys | Yes |
P4-G7-SP6 | pP4-G7 | 2,599 | depC, depD | AmphI (AAK73501), NosC (AAF17280) | KRi-ACP, C-Ai | PKS, NRPS | NAd | NA | Yes |
See Fig. 2.
A superscript i indicates incomplete. KS, β-ketoacyl synthase; E, epimerase.
NN, not named.
NA, not available.