TABLE 2.

Properties of three sequence tags and their associated candidate (partial) genes

Sequence tag	Recombinant plasmid	Insert size (bp)	Associated gene(s)a	Protein homolog(s) (accession no.)	Domain organizationb	Protein classification	Signature motif(s) or substrate specificity	Predicted substrate specificity	Necessary for FK228 biosynthesis
P3-A6-SP6	pP3-A6	2,826	NNc	JamL (AAS98783)	KS-ATi	Type I PKS	QTRTAQ, GHSYG, and AAFH in AT domain	MCoA	No
P4-B4-SP6	pP4-B4	3,612	depD	BmyB (CAE11249)	Ci-A-PCP-E	Type A NRPS	DLFEMSLIWK in A domain	l-Cys	Yes
P4-G7-SP6	pP4-G7	2,599	depC, depD	AmphI (AAK73501), NosC (AAF17280)	KRi-ACP, C-Ai	PKS, NRPS	NAd	NA	Yes

^aSee Fig. 2.

^bA superscript i indicates incomplete. KS, β-ketoacyl synthase; E, epimerase.

^cNN, not named.

^dNA, not available.