TABLE 3.
Deduced functions of open reading frames and genes in the dep gene cluster and flanking regions
| Open reading frame or gene | Protein size (amino acids) | Protein homolog | Accession no. | % Identity/ % similarity | Origin | Proposed functiona |
|---|---|---|---|---|---|---|
| orf1b | 150c | CV_3386 | AAQ61050 | 87/93 | C. violaceum ATCC 12472 | 16S rRNA pseudouridine synthase |
| orf2 | 163 | CV_3385 | AAQ61049 | 66/76 | C. violaceum ATCC 12472 | MutT/nudix family phosphohydrolase |
| orf3 | 190 | CV_3384 | AAQ61048 | 88/94 | C. violaceum ATCC 12472 | Transcription elongation factor GreB |
| depK | 85 | CCO_1235 | EAL57087 | 36/52 | Campylobacter coli RM2228 | Conserved hypothetical protein, function unknown |
| depL | 155 | CV_3383 | AAQ61047 | 68/78 | C. violaceum ATCC 12472 | Helix-turn-helix transcriptional regulator, MarR family |
| depM | 389 | PFL_4362 | AAY93617 | 59/73 | Pseudomonas fluorescens Pf-5 | Aminotransferase, class I and II family protein |
| depN | 65 | PCPn | ||||
| depA | 1,697 | SafB | AAC44128 | 31/45 | Myxococcus xanthus strain Mx ×48 | NRPS: ALi-Cn-ACys-PCP |
| depB | 1,553 | CurG | AAT70102 | 45/61 | Lyngbya majuscula | PKS: KS-ATn-DHn-KRi-ACP |
| depC | 1,183 | CrpB | ABM21570 | 44/64 | Nostoc sp. strain ATCC 53789 | PKS: KS-DHn-KRi-ACP |
| depD | 3,057 | PvdI | AAX16361 | 36/51 | Pseudomonas aeruginosa | NRPS: C-AVal-PCP-E-C-ACys-PCP-E |
| depE | 1,892 | McyB | BAA83993 | 35/52 | Microcystis aeruginosa | NRPS: C-ADhb-PCP-C-PCP-TE |
| depF | 390 | PP_2437 | AAN68049 | 38/56 | Pseudomonas putida KT2440 | FadE2-like acyl coenzyme A dehydrogenase |
| depG | 321 | PSPTO_2724 | AAO56225 | 32/53 | Pseudomonas syringae pv. tomato DC3000 | Phosphotransferase |
| depH | 319 | PA4170 | AAG07557 | 56/70 | Pseudomonas aeruginosa PAO1 | Flavin adenine dinucleotide-dependent pyridine nucleotide-disulfide oxidoreductase |
| depI | 304 | RRSL_03772 | EAP73858 | Ralstonia solanacearum UW551 | Putative esterase/lipase | |
| depJ | 254 | LnmN | AAN85527 | 43/58 | Streptomyces atroolivaceus S-140 | Type II thioesterase |
| orf18 | 312 | CV_3378 | AAQ60142 | 87/93 | C. violaceum ATCC 12472 | Hydrogen peroxide-inducible gene activator OxyR |
| orf19 | 85 | CV_3377 | AAQ60141 | 92/98 | C. violaceum ATCC 12472 | Cell division topological specificity factor MinE |
| orf20 | 270 | CV_3376 | AAQ61040 | 92/98 | C. violaceum ATCC 12472 | Septum site-determining protein MinD |
| orf21b | 107c | CV_3375 | AAQ61039 | 93/97 | C. violaceum ATCC 12472 | Septum formation inhibitor MinC |
a
Subscripts indicate the substrate specificities of enzymes. Superscripts indicate inactive (i) or nonfunctional (n). Dhb, 2,3-dehydro-2-aminobutanoic acid.
b
Incomplete.
c
Truncated.