TABLE 2.
Primers used in gel mobility shift assays and primer extension analysis
| Primer | Sequence (5′ to 3′) | Position (nt)a |
|---|---|---|
| rpoS-1 | CAAGTCTCCTACGGGTTGGACCTG | −117 to −94 |
| rpoS-2 | TGTGTTTGATGATGATCAAATCGC | +77 to +54 |
| rpoS-3 | ACTAACAACCTGCGATTATGACCG | +123 to +100 |
| aefR-1 | ATATGTAACAGTGTTTTACACTAC | −76 to −53 |
| aefR-2 | TGTTCTAGATGGCCGCCTGGACGATGGACTCG | +94 to +71 |
| psrA-1 | CCTTTGTCAGGTAGCTTGTATAAG | −122 to −99 |
| psrA-2 | TGTTCTAGAGCAGCATCAAGAATGCGTTCAACGG | +65 to +41 |
| psrA-3 | CGCAAGGATGTTTCAGCGAAACC | +110 to +88 |
a
Corresponds to base positions relative to the transcriptional start site.