TABLE 3.
Expression of psrA-lacZ, aefR-lacZ, and rpoS-lacZ fusions in DC3000 and its PsrA−, AefR−, and RpoS− mutants
| Bacterial constructa | Relevant characteristic | β-Galactosidase activity (mean ± SD)b |
|---|---|---|
| DC3000(pMP220) | Wild type (vector) | 31 ± 3 |
| AC820(pMP220) | PsrA− (vector) | 29 ± 4 |
| AC821(pMP220) | AefR− (vector) | 34 ± 6 |
| AC822(pMP220) | RpoS− (vector) | 32 ± 5 |
| DC3000(pAKC1257) | Wild type (psrA-lacZ) | 1,766 ± 70 |
| AC820(pAKC1257) | PsrA− (psrA-lacZ) | 5,391 ± 117 |
| AC821(pAKC1257) | AefR− (psrA-lacZ) | 1,713 ± 100 |
| AC822(pAKC1257) | RpoS− (psrA-lacZ) | 1,745 ± 111 |
| DC3000(pAKC1258) | Wild type (aefR-lacZ) | 800 ± 49 |
| AC820(pAKC1258) | PsrA− (aefR-lacZ) | 1,396 ± 47 |
| AC822(pAKC1258) | RpoS− (aefR-lacZ) | 848 ± 42 |
| DC3000(pAKC1259) | Wild type (rpoS-lacZ) | 1,430 ± 49 |
| AC820(pAKC1259) | PsrA− (rpoS-lacZ) | 263 ± 13 |
| AC821(pAKC1259) | AefR− (rpoS-lacZ) | 1,413 ± 61 |
a
Bacteria were grown in KB medium plus tetracycline to a Klett value of ca. 600, and the cultures were used for assays.
b
Expressed as Miller units.