TABLE 5.
Expression of psyI-lacZ and psyR-lacZ in DC3000 and its PsrA−, AefR−, and RpoS− mutants
| Bacterial constructa | Relevant characteristic | β-Galactosidase activity (mean ± SD)b |
|---|---|---|
| DC3000(pMP220) | Wild type (vector) | 29 ± 3 |
| AC820(pMP220) | PsrA− (vector) | 27 ± 4 |
| AC821(pMP220) | AefR− (vector) | 32 ± 4 |
| AC822(pMP220) | RpoS− (vector) | 32 ± 4 |
| DC3000(pAKC1255) | Wild type (psyI-lacZ) | 789 ± 6 |
| AC820(pAKC1255) | PsrA− (psyI-lacZ) | 1,468 ± 14 |
| AC821(pAKC1255) | AefR− (psyI-lacZ) | 510 ± 12 |
| AC822(pAKC1255) | RpoS− (psyI-lacZ) | 1,213 ± 14 |
| DC3000(pAKC1256) | Wild type (psyR-lacZ) | 215 ± 12 |
| AC820(pAKC1256) | PsrA− (psyR-lacZ) | 398 ± 13 |
| AC821(pAKC1256) | AefR− (psyR-lacZ) | 148 ± 12 |
| AC822(pAKC1256) | RpoS− (psyR-lacZ) | 311 ± 13 |
a
Bacterial cultures used for assays were grown in KB medium plus tetracycline to a Klett value of ca. 600.
b
Expressed as Miller units.