FIG. 3.

Lipolytic activity (left axis) in culture supernatants (white bars) and relative fluorescence measurements (relative fluorescence units [RFU]) (right axis) of transcriptional (pGPG1-TC and pGLU-TC; gray bars) or translational (pGPG1-TL and pGLU-TL; black bars) lipA::gfp fusions in B. glumae PG1 (A) or LU8093 (B). Strains were grown for 24 h in PG medium supplemented with 1% olive oil (oil), 1% olive oil and 10% hexadecane (oil+HD), 0.5% sucrose (sucrose), or 0.5% sucrose and 0.1% Tween 80 (sucrose+Tw). Averages from at least three experiments are shown, with error bars indicating standard deviations. Asterisks indicate significant difference from sample without hexadecane or Tween 80 (P < 0.05).