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R. solanacearum Tat gene organization (GMI1000 genome). To create a Tat secretion mutant of R. solanacearum, a kanamycin resistance gene cassette (open triangle) was inserted into tatC, a gene predicted to encode an essential component of the Tat secretion system. R. solanacearum tatC is predicted to encode a 267-amino-acid protein with a molecular mass of 29.7 kDa. The entire tatABC gene cluster was used for all trans complementation studies of the R. solanacearum tatC mutant, K60TAT.
