TABLE 1.
Strains and plasmids used in this research
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Escherichia coli strain | ||
| DH5α | F−endA1 relA φ80lacZΔ supE44 thi-1 recA1 gyrA96 | 16 |
| Ralstonia solanacearum strains | ||
| GMI1000 | Wild-type phylotype I, biovar 3 | 4 |
| K60 | Wild-type phylotype II, biovar 1 | 25 |
| K60-409 | pehC::aphA-3; Kmr | 15 |
| K60TAT | tatC::pTatC(s); Kmr | This study |
| K1575 | RSp1575::aacC1; Gmr | This study |
| K1651 | RSc1651::aacC1; Gmr | This study |
| K1521 | RSp1521::aacC1; Gmr | This study |
| Plasmids | ||
| pSTblue-1 | Kmr Apr | Novagen |
| pLAFR3 | Tcr | 38 |
| pUCGM | Gmr Apr | 36 |
| pCR-1 | Kmr Zeor | Epicenter |
| pTatC(s) | 533-bp internal tatC fragment cloned into pCR-1; Kmr Zeor | This study |
| pSTTAT | 1.8-kb insert containing tatABC in pSTblue-1; Kmr Apr | |
| pLTat | 1.8-kb insert containing tatABC in pLAFR3; Tetr | This study |
| pST2131 | 701-bp internal RSp1575 fragment cloned into pSTblue-1; Kmr Apr | This study |
| pST2131Gm | pST2131 with 850-bp Gmr cassette insertion from pUCGM; Kmr Apr Gmr | This study |
| pST4028 | 1,185-bp insert containing RSc1651 in pSTblue-1; Kmr Apr Gmr | This study |
| pST4048Gm | pST4028 with 850-bp Gmr cassette insertion from pUCGM; Kmr Apr Gmr | This study |
| pST4798 | 1,809-bp insert containing RSp1521 in pSTblue-1; Kmr Apr | This study |
| pST4798Gm | pST4798 with 850-bp Gmr cassette insertion from pUCGM; Km Kmr Apr Gmr | This study |
| pLPehCRRKK | 3.2-kb insert in pLAFR3 containing R25K and R26K mutations PehC; Tetr | This study |
| pPehC11 | 3.8-kb BalI-Alw44I fragment containing pehC-exuT in pLAFR3; Tcr | 15 |
a
Ap, ampicillin; Tc, tetracycline; Km, kanamycin; Gm, gentamicin; Zeo, zeocin.